Open AccessAnti-inflammatory potentials, membrane stabilizing and xanthine oxidase inhibitory activities of Clerodendrum volibule ethanolic leaf extract on carragenaan- induced inflammation in rats
Author(s) -
Olarenwaju Olufunmilayo.,
O. Uguru Mary,
Oyemitan Idris,
Akomolafe Rufus,
Akinpelu Bola,
A. Aderonmu Joseph,
Iwalewa Ezekiel Olugbenga
Publication year - 2018
Publication title -
international journal of pharmacology and toxicology
Language(s) - English
Resource type - Journals
ISSN - 2310-2985
DOI - 10.14419/ijpt.v6i1.8410
Subject(s) - xanthine oxidase , inflammation , inhibitory postsynaptic potential , chemistry , pharmacology , traditional medicine , biochemistry , biology , enzyme , medicine , immunology , endocrinology
The folkloric use of Clerodendrum volubile P Beauv (Verbenaceae) for treatment of inflammatory conditions in the Southern part of Nigeria has been reported. The anti-inflammatory action of the ethanolic leaf extract on carrageenan-induced rat paw oedema, lipoxygenase and xanthine oxidase inhibitory effects, and membrane stabilizing potential were evaluated. The extract reduced carrageenan-induced rat paw oedema in a dose dependent manner compared to control group. C. volubile inhibited xanthine oxidase activity in a dose - dependent manner; at 0.5 mg/ml it exhibited maximum inhibitory activity of 95.48% while the standard drug, allopurinol exhibited 70 % inhibition. The extract significantly inhibited lipoxygenase activity, with highest activity at 0.4 mg/ml while Quercetin showed maximum inhibition of the enzyme at 0.1 mg/ml. The extract and Acetaminophen exhibited maximum membrane stabilizing activity of 91.85 ± 0.50% and 94.32 ± 0.32% at 2.5 mg/ml respectively. These findings provide justification for the traditional use of C. volubile in inflammatory conditions.