Open AccessFractionation of Sugarcane Extracts and Determination of Phenolic Compounds and their Antioxidant Activity
Author(s) -
Kittisak Kerdchan,
Prasong Srihanam
Publication year - 2022
Publication title -
asian journal of chemistry/asian journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.145
H-Index - 34
eISSN - 0975-427X
pISSN - 0970-7077
DOI - 10.14233/ajchem.2022.23474
Subject(s) - chemistry , myricetin , rutin , gallic acid , phytochemical , quercetin , antioxidant , chromatography , flavonoid , fractionation , catechin , saccharum officinarum , high performance liquid chromatography , flavonols , phenols , polyphenol , food science , botany , biochemistry , kaempferol , biology
Crude ethanolic extracts of four cultivars of sugarcane (Saccharum officinarum L.) were firstly preparedand then fractionated using silica gel column chromatography before the determination of their oxidativesubstances. The fractionated extracts were then tested for antioxidant activity by various assays. Finally,high-performance liquid chromatography (HPLC) was applied for the quantitative determination ofthe individual phenolic compounds. Sub-fraction 2 has the highest phytochemical contents as well asantioxidant activity. All tested phytochemicals had positively correlated to antioxidant activity. HPLCanalysis showed that the phytochemicals in the fractionated extracts varied by the sugarcane cultivars.The main flavonoid substances found in the fractionated extracts were epicatechin, catechin, quercetin,resveratrol, myricetin and rutin while gallic acid was the main phenolic substance. The obtainedinformation is useful for further studies and applications.