Open AccessMicrobial Inactivation in the Liquid Phase Induced by Multigas Plasma Jet
Author(s) -
Takeichiro Takamatsu,
Kodai Uehara,
Yota Sasaki,
Hidekazu Miyahara,
Yukihiko Matsumura,
Atsuo Iwasawa,
Norihiko Ito,
Masahiro Kohno,
Takeshi Azuma,
Akitoshi Okino
Publication year - 2015
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0132381
Subject(s) - singlet oxygen , sterilization (economics) , bacteria , radical , chemistry , carbon dioxide , plasma , microorganism , nitrogen , oxygen , spore , atmospheric pressure plasma , gas phase , microbiology and biotechnology , environmental chemistry , biochemistry , biology , organic chemistry , physics , quantum mechanics , monetary economics , economics , foreign exchange market , genetics , foreign exchange
Various gas atmospheric nonthermal plasmas were generated using a multigas plasma jet to treat microbial suspensions. Results indicated that carbon dioxide and nitrogen plasma had high sterilization effects. Carbon dioxide plasma, which generated the greatest amount of singlet oxygen than other gas plasmas, killed general bacteria and some fungi. On the other hand, nitrogen plasma, which generated the largest amount of OH radical, killed ≥6 log of 11 species of microorganisms, including general bacteria, fungi, acid-fast bacteria, spores, and viruses in 1–15 min. To identify reactive species responsible for bacterial inactivation, antioxidants were added to bacterial suspensions, which revealed that singlet oxygen and OH radicals had greatest inactivation effects.