Open AccessAdaptive optics allows STED-FCS measurements in the cytoplasm of living cells
Author(s) -
Aurélien Barbotin,
Silvia Galiani,
Iztok Urbančič,
Christian Eggeling,
Martin J. Booth
Publication year - 2019
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.27.023378
Subject(s) - sted microscopy , optics , adaptive optics , physics , stimulated emission , laser
Fluorescence correlation spectroscopy in combination with super-resolution stimulated emission depletion microscopy (STED-FCS) is a powerful tool to investigate molecular diffusion with sub-diffraction resolution. It has been of particular use for investigations of two dimensional systems like cell membranes, but has so far seen very limited applications to studies of three-dimensional diffusion. One reason for this is the extreme sensitivity of the axial (z) STED depletion pattern to optical aberrations. We present here an adaptive optics-based correction method that compensates for these aberrations and allows STED-FCS measurements in the cytoplasm of living cells.