Open AccessEndothelial-like nitric oxide synthase immunolocalization by using gold nanoparticles and dyes
Author(s) -
Ramla Gary,
Daniela Amelio,
Filippo Garofalo,
Gia Petriashvili,
Maria Penelope De Santo,
Yuen K. Ip,
R. Barberi
Publication year - 2015
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.6.004738
Subject(s) - confocal , fluorescence , confocal microscopy , nitric oxide synthase , colloidal gold , immunofluorescence , microscopy , fluorescence microscope , fluorescein , molecular imaging , nanoparticle , biophysics , materials science , chemistry , fluorescence lifetime imaging microscopy , microscope , nitric oxide , biomedical engineering , nanotechnology , microbiology and biotechnology , pathology , optics , in vivo , biology , medicine , antibody , immunology , physics , organic chemistry
Immunofluorescence is a biological technique that allows displaying the localization of the target molecule through a fluorescent microscope. We used a combination of gold nanoparticles and the fluorescein isothiocianate, FITC, as optical contrast agents for laser scanning confocal microscopy imaging to localize the endothelial-like nitric oxide synthase in skeletal muscle cells in a three-dimensional tissue phantom at the depth of 4µm. The FITC detected fluorescence intensity from gold-nanoparticles-labelled cells was brighter than the emission intensity from unlabelled cells.