Open AccessFast hybrid optomechanical scanning photoacoustic remote sensing microscopy for virtual histology
Author(s) -
Brendon S. Restall,
Brendyn D. Cikaluk,
Matthew T. Martell,
Nathaniel J. M. Haven,
Rohan Mittal,
Sveta Silverman,
Lashan Peiris,
Jean Deschênes,
Benjamin Adam,
Adam Kinnaird,
Roger J. Zemp
Publication year - 2021
Publication title -
biomedical optics express
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.443751
Subject(s) - galvanometer , histology , microscopy , biomedical engineering , h&e stain , photoacoustic imaging in biomedicine , materials science , pathology , medicine , staining , nuclear medicine , optics , laser , physics
A rapid scanning microscopy method for hematoxylin and eosin (H&E) like images is sought after for interoperative diagnosis of solid tumor margins. The rapid observation and diagnosis of histological samples can greatly lower surgical risk and improve patient outcomes from solid tumor resection surgeries. Photoacoustic remote sensing (PARS) has recently been demonstrated to provide images of virtual H&E stains with excellent concordance with true H&E staining of formalin-fixed, paraffin embedded tissues. By using PARS with constant velocity and 1D galvanometer mirror scanning we acquire large virtual H&E images (10mm x 5mm) of prostate tissue in less than 3.5 minutes without staining, and over two orders of magnitude faster data acquisition than the current PARS imaging speed.