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Type B haemophilus influenzae —induced otitis media in the mouse
Author(s) -
Krekorian Terry D.,
Keithley Elizabeth M.,
Harris Jeffrey P.,
Fierer Joshua
Publication year - 1991
Publication title -
the laryngoscope
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.181
H-Index - 148
eISSN - 1531-4995
pISSN - 0023-852X
DOI - 10.1288/00005537-199106000-00013
Subject(s) - haemophilus influenzae , otitis , middle ear , cytotoxic t cell , antibody , immunology , immune system , medicine , microbiology and biotechnology , biology , in vitro , anatomy , biochemistry , surgery , antibiotics
Abstract To characterize the middle and inner ear cellular inflammatory responses to otitis media using im‐munohistochemical methods, we inoculated type B Haemophilus influenzae into the middle ears of healthy adult BALB/c mice. Mac‐1 + neutrophils and macrophages appeared in the middle ear at 3 days. Lyt‐1 + T cells and Lyt‐2 + T suppressor/cytotoxic cells entered the middle ear mucosa on days 7 and 14. IgG + and IgM + T cells were present at all time points, with IgA + lymphocytes forming the majority of mucosal immunoglobulin‐bearing cells at 2 weeks. The co‐chlear scala tympani contained Lyt‐1 + and Mac‐1 + cells and two endolymphatic sacs stained diffusely with anti‐IgA and ‐IgG antibodies. Lyt‐1/L3T4 + T lymphocytes greatly outnumbered B lymphocytes, suggesting that helper/inducer T cells play a more important role in acute otitis media than has been recognized. Inner ear changes occurred after a single episode of otitis media.