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Involvement of TNF‐Related Apoptosis‐Inducing Ligand (TRAIL) Induction in Interferon γ‐Mediated Apoptosis in Ewing Tumor Cells
Author(s) -
ABADIE ANNIE,
WIETZERBIN JUANA
Publication year - 2003
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1299.020
Subject(s) - apoptosis , autocrine signalling , paracrine signalling , tumor necrosis factor alpha , interferon gamma , cancer research , programmed cell death , cytokine , cell , uvb induced apoptosis , immunology , biology , medicine , receptor , caspase , biochemistry
A bstract : We investigated the effect of IFN γ or IFN γ/IFN α and IFN γ/TNF treatment on the apoptosis of Ewing tumor cells (SK‐N‐MC). The results show that treatment of cells with IFN γ resulted in 17% of cell death while no effect was observed when cells were treated with IFN α or TNF alone. Percentage cell death increased markedly in IFN γ/IFN α‐ and IFN γ/TNF‐treated cells (42% and 67%, respectively) as compared to IFN γ‐treated cells. These results suggest that IFN α and TNF amplified the apoptotic signal(s) induced by IFN γ. It was shown recently that Ewing cells undergo apoptosis upon treatment with recombinant TRAIL (TNF‐related apoptosis inducing ligand), a member of the TNF family. Thus, since cytokines were reported to be able to induce TRAIL in other cell systems, we were prompted to investigate whether TRAIL induction was involved in the mechanism responsible for IFN γ‐mediated cell death in Ewing cells. The results reported here are consistent with the notion that cell‐associated and secreted TRAIL contribute in an autocrine or paracrine manner to the triggering of Ewing cell apoptosis induced by IFN γ alone or combined with IFN α or TNF. The observations reported here might contribute to the development of alternative new approaches to the treatment of Ewing tumors resistant to conventional therapy.