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LPS induces rapid IL‐10 release by M‐CSF‐conditioned tolerogenic dendritic cell precursors
Author(s) -
Kwan WingHong,
Boix Charlotte,
Gougelet Nicolas,
Fridman Wolf H.,
Mueller Chris G. F.
Publication year - 2007
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1189/jlb.0406267
Subject(s) - dendritic cell , biology , immune system , microbiology and biotechnology , t cell , inflammation , immunology
Dendritic cells (DC) obtained by culturing myeloid precursors in GM‐CSF undergo maturation and induce an efficient T cell response when stimulated with microbial products. DC precursors themselves also recognize microbial products, and it remains unclear how these stimulated DC precursors modulate the immune response. We show here that M‐CSF‐conditioned human DC precursors responded to LPS, Mycobacteria bovis , and inflammatory cytokines by a rapid and robust production of IL‐10, largely superior to that observed with immature DC or monocytes. The endogenous IL‐10 restrained the DC precursors from converting into professional APC, as blocking the IL‐10 receptor in the presence of LPS resulted in the formation of efficient T cell stimulators. LPS stimulation concomitant with DC differentiation gave rise to immature DC, which were tolerant to a secondary LPS exposure. Furthermore, the LPS‐activated DC precursors reduced bystander DC maturation and anti‐CD3/CD28‐triggered T cell activation. These data suggest that when exposed to inflammatory or microbial signals, M‐CSF‐conditioned DC precursors can participate in the modulation of inflammation and immune response by rapid release of IL‐10.