Premium
Direct quantification of the modulation of interaction between cell‐ or surface‐bound LFA‐1 and ICAM‐1
Author(s) -
Vitte Joana,
Pierres Anne,
Benoliel AnneMarie,
Bongrand Pierre
Publication year - 2004
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1189/jlb.0204077
Subject(s) - cell adhesion , avidity , jurkat cells , surface plasmon resonance , cell adhesion molecule , biophysics , adhesion , integrin , intercellular adhesion molecule 1 , cell–cell interaction , lymphocyte function associated antigen 1 , receptor , intracellular , biology , cell surface receptor , microbiology and biotechnology , intercellular adhesion molecule , cell , t cell , chemistry , biochemistry , antigen , nanotechnology , materials science , immunology , immune system , nanoparticle , organic chemistry
The functional activity of leukocyte integrins is highly regulated by several mechanisms related to intrinsic molecular properties and receptor interaction with the cell membrane. Here, we present a microkinetic study of the lymphocyte function‐associated antigen‐1‐mediated interaction between flowing Jurkat cells and surface‐ or cell‐bound intercellular adhesion molecule‐1 (ICAM‐1). We conclude that adhesion is initiated by the formation of a single bond with ∼0.3 s –1 dissociation rate, and attachment is subsequently strengthened by the formation of additional bonds during the next 10 s; exposing cells to Mg 2+ or Mn 2+ resulted in up to a 16‐fold increase of the binding frequency, in line with reported measurements performed on isolated molecules with surface plasmon resonance methodology; cell‐bound ICAM‐1 molecules were more efficient in mediating adhesion than Fc‐ICAM‐1, properly oriented and bound by surface‐adsorbed protein A; and quantitative analysis of binding frequency suggested that adhesion efficiency was ten‐ to 100‐fold lower than the maximum value allowed by previously determined association rates of soluble molecules. It is concluded that the presented methodology provides a simple and unique way of dissecting the initial step of cell adhesion and discriminating between affinity and avidity modulation of adhesion receptors.