Open AccessA novel approach for characterisation of conformational allergen epitopes combining phage display and high‐throughput sequencing
Author(s) -
Christiansen Anders,
Hansen Christian Skjodt,
Kringelum Jens Vindahl,
Lund Ole,
Bogh Katrine Lindholm,
Dufva Martin
Publication year - 2014
Publication title -
clinical and translational allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.979
H-Index - 37
ISSN - 2045-7022
DOI - 10.1186/2045-7022-4-s2-p27
Subject(s) - epitope , allergen , conformational epitope , computational biology , epitope mapping , phage display , medicine , allergy , antibody , immunology , biology
Background Characterization of allergen epitopes is an essential task in unraveling the molecular mechanisms of allergy. At present, the procedures to characterize conformational epitopes are relatively laborious and, consequently, knowledge of conformational allergen epitopes is limited. The aim of this study was to couple an established epitope mapping approach, based on phage-displayed peptide libraries, with high-throughput sequencing in order to make conformational epitope mapping less laborious and increase data output.