Open AccessA High-Performance Liquid Chromatography Method for Determination of 4-Aminopyridine in Tissues and Urine
Author(s) -
Stan W. Casteel,
Brian Thomas
Publication year - 1990
Publication title -
journal of veterinary diagnostic investigation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.529
H-Index - 78
eISSN - 1943-4936
pISSN - 1040-6387
DOI - 10.1177/104063879000200210
Subject(s) - chemistry , chromatography , urine , high performance liquid chromatography , phosphoric acid , 2 aminopyridine , aqueous solution , sodium , acetonitrile , biochemistry , organic chemistry
An analytical method was developed to measure 4-aminopyridine in tissues and urine to determine appropriate diagnostic samples in acute poisoning cases. Tissues from rats dosed with 4-aminopyridine were extracted with methylene chloride. Extracts were analyzed by high-performance liquid chromatography using an isocratic solvent system of acetonitrile and aqueous solution (15/85 v/v) consisting of 0.015 M sodium salt of 1-heptane-sulfonic acid, 0.002 M tetramethylammonium bromide, and 0.01 M sodium dihydrogen phosphate adjusted to pH 3.0 with phosphoric acid. We concluded that suitable diagnostic samples for acute poisoning cases include stomach contents, kidney, liver, and urine.
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