Open AccessThe HPV16 E1 Carboxyl Domain Provides a Helper Function for Adeno-Associated Virus Replication
Author(s) -
Ming Cao,
Sarmistha Bandyopadhyay,
Hongqing Zhu,
Hong You,
Paul L. Hermonat
Publication year - 2018
Publication title -
intervirology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.641
H-Index - 61
eISSN - 1423-0100
pISSN - 0300-5526
DOI - 10.1159/000495137
Subject(s) - microbiology and biotechnology , plasmid , biology , complementary dna , adeno associated virus , recombinant dna , dna replication , transfection , helper virus , virology , gene , viral replication , virus , vector (molecular biology) , genetics
Recombinant adeno-associated virus (rAAV) is now in the clinic, yet production of rAAV remains problematic. We previously determined that human papillomavirus type 16 (HPV16) E1 protein boosts rAAV yields and E1 enhances AAV Rep78's replication-related biochemistries. Here, we deletion-mapped the helper domain within E1 to help glean its mechanism of action.