Open AccessMonitoring of cAMP Synthesis and Degradation in Living Cells
Author(s) -
Viacheslav O. Nikolaev,
Martin J. Lohse
Publication year - 2006
Publication title -
physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.14
H-Index - 125
eISSN - 1548-9213
pISSN - 1548-9221
DOI - 10.1152/physiol.00057.2005
Subject(s) - second messenger system , effector , protein kinase a , robustness (evolution) , cyclic nucleotide , microbiology and biotechnology , kinase , biology , chemistry , electrophysiology , biophysics , signal transduction , nucleotide , computational biology , biochemistry , neuroscience , gene
cAMP is an important second messenger with a plethora of cellular effects and biological roles. To monitor and visualize cAMP in intact living cells, electrophysiological and fluorescent methods have been developed based on activation of all three types of cAMP effectors: protein kinase A, cyclic nucleotide-gated channels, and exchange protein directly activated by cAMP. In this review, we describe and compare these techniques in terms of their robustness, sensitivity and spatio-temporal resolution.
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