Open AccessIn vivo evaluation of laser-induced choroidal neovascularization in rats simultaneously using optical coherence tomography and photoacoustic microscopy
Author(s) -
Fengxian Du,
Lei Gao,
Lin Li,
Qian Li,
Fenghua Wang,
Chuanqing Zhou,
Cuixia Dai
Publication year - 2021
Publication title -
journal of innovative optical health sciences/journal of innovation in optical health science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.421
H-Index - 24
eISSN - 1793-5458
pISSN - 1793-7205
DOI - 10.1142/s1793545821400125
Subject(s) - choroidal neovascularization , optical coherence tomography , in vivo , fundus (uterus) , medicine , laser , macular degeneration , ophthalmology , contrast (vision) , h&e stain , neovascularization , preclinical imaging , biomedical engineering , pathology , optics , staining , angiogenesis , biology , physics , microbiology and biotechnology
Determination of the precise location and the degree of the Choroidal neovascularization (CNV) lesion is essential for diagnosation Neovascular age-related macular degeneration (AMD) and evaluation the efficacy of treatment. Noninvasive imaging techniques with specific contrast for CNV evaluation are demanded. In this paper, two noninvasive imaging techniques, namely Optical coherence tomography (OCT) and Photoacoustic microscopy (PAM), are combined to provide specific detection of CNV for their complimentary contrast mechanisms. In vivo time-serial evaluation of Laser-induced CNV in rats is present at days 1, 3, 5, 7, 14, 21 after laser photocoagulation is applied to the rat fundus. Both OCT and PAM show that the CNV increases to its maximum at day 7 and decreases at day 14. Quantification of CNV area and CNV thickness is given. The dual-modal information of CNV is consistent with the histologic evaluation by hematoxylin and eosin (H&E) staining.