Open AccessReplication factor A is required in vivo for DNA replication, repair, and recombination.
Author(s) -
Maria Pia Longhese,
Paolo Plevani,
Giovanna Lucchini
Publication year - 1994
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.14.12.7884
Subject(s) - biology , replication factor c , replication protein a , pre replication complex , dna replication , control of chromosome duplication , eukaryotic dna replication , origin recognition complex , dna polymerase delta , dna polymerase ii , dna polymerase , dna clamp , genetics , primase , dna replication factor cdt1 , dna repair , microbiology and biotechnology , dna , dna binding protein , gene , transcription factor , polymerase chain reaction , reverse transcriptase
Replication factor A (RF-A) is a heterotrimeric single-stranded-DNA-binding protein which is conserved in all eukaryotes. Since the availability of conditional mutants is an essential step to define functions and interactions of RF-A in vivo, we have produced and characterized mutations in the RFA1 gene, encoding the p70 subunit of the complex in Saccharomyces cerevisiae. This analysis provides the first in vivo evidence that RF-A function is critical not only for DNA replication but also for efficient DNA repair and recombination. Moreover, genetic evidence indicate that p70 interacts both with the DNA polymerase alpha-primase complex and with DNA polymerase delta.