Open AccessImmunoglobulin G- and M-specific enzyme-linked immunosorbent assay for detection of dengue antibodies
Author(s) -
David Dittmar,
Thomas P. Cleary,
Albert Castro
Publication year - 1979
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.9.4.498-502.1979
Subject(s) - antibody , dengue virus , dengue fever , hemagglutination assay , immunoglobulin g , virology , immunoglobulin m , enzyme , biology , hemagglutination , microbiology and biotechnology , immunology , titer , biochemistry
An enzyme-linked immunosorbent assay for the detection of antibodies to dengue virus is described. This method correlates well with a hemagglutination inhibition technique. The enzyme-linked immunosorbent assay can also be specific for human immunoglobulin M antibodies when a mu-chain-specific antiglobulin-enzyme conjugate and fractionated serum are employed. By using this technique, dengue immunoglobulin M antibodies were demonstrated in an infant suspected of having a recent dengue infection.