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We have performed the fluorescently labeled amplified-fragment length polymorphism (FAFLP) method on 97 strains of the food-borne pathogenSalmonella enterica subsp.enterica comprising seven different serovars using the restriction enzymesEco RI andMse I. From the total FAFLP fingerprinted strains, 81 were compared with pulsed-field gel electrophoresis (PFGE) typing of the same strains. The FAFLP method showed a discriminatory power equal to that of PFGE. We report a fast, robust, and high-resolution adaptation of the AFLP assay for fingerprintingS. enterica subsp.enterica serovars with capillary electrophoresis that can be scaled to high throughput on automated analysis instruments.

journal of clinical microbiology

Fluorescent Amplified-Fragment Length Polymorphism Genotyping of <i>Salmonella enterica</i> subsp. <i>enterica</i> Serovars and Comparison with Pulsed-Field Gel Electrophoresis Typing

Fluorescent Amplified-Fragment Length Polymorphism Genotyping of Salmonella enterica subsp. enterica Serovars and Comparison with Pulsed-Field Gel Electrophoresis Typing