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To study possible nosocomial transmission of multidrug-resistant (MDR)Mycobacterium tuberculosis , strain types and other information on 24, mostly human immunodeficiency virus-positive patients, were collected. Isolates from 11 patients had identical IS6110 restriction fragment length polymorphism (RFLP) patterns as well as spoligotype patterns and resistance profiles. Noticeably, nine other isolates from related cases also exhibited identical spoligotypes but slightly different RFLP patterns. These results indicate that for some MDR strains, the evolutionary clock of IS6110 RFLP may run too fast for reliable interpretation of strain typing results over a period of a few years.

journal of clinical microbiology

The IS <i>6110</i> Restriction Fragment Length Polymorphism in Particular Multidrug-Resistant <i>Mycobacterium tuberculosis</i> Strains May Evolve Too Fast for Reliable Use in Outbreak Investigation

The IS 6110 Restriction Fragment Length Polymorphism in Particular Multidrug-Resistant Mycobacterium tuberculosis Strains May Evolve Too Fast for Reliable Use in Outbreak Investigation