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Recently a novel insertion element, IS1245 , has been described and suggested for use as a probe in restriction fragment length polymorphism studies ofMycobacterium avium strains. An important issue in this context is the stability of the insertion element. We analyzed single colonies ofM. avium cultures and found frequent small one- to two-band changes. However, following repeated in vitro passages over 1 year, similar one- to two-band changes were observed in the IS1245 patterns of only sixM. avium strains investigated.

Stability of Insertion Sequence IS 1245 , a Marker for Differentiation of Mycobacterium avium Strains