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A PCR was developed for the detection ofEscherichia coli O157 based on therfbE O-antigen synthesis genes. A 479-bp PCR product was amplified specifically fromE. coli O157 in cell lysates containing 200 or 2 CFU following crude DNA extraction. The PCR detected &lt;1 CFU ofE. coli O157 per ml in raw milk following enrichment.

A PCR Specific for Escherichia coli O157 Based on the rfb Locus Encoding O157 Lipopolysaccharide