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Primers were designed to amplify sequences of verocytotoxin genes andeaeA genes ofEscherichia coli O26:H11, O111:H8, and O157:H7 in a multiplex PCR assay. This assay successfully detectedE. coli O26:H11 in bloody stool specimens in which other enteric pathogens were not detected by culture-based methods. Rapid assays to detect non-O157:H7 verocytotoxin-producingE. coli is important to improve methods for the etiologic diagnosis of hemorrhagic colitis.

journal of clinical microbiology

Application of Multiplex PCR for Detection of Non-O157 Verocytotoxin-Producing <i>Escherichia coli</i> in Bloody Stools: Identification of Serogroups O26 and O111

Application of Multiplex PCR for Detection of Non-O157 Verocytotoxin-Producing Escherichia coli in Bloody Stools: Identification of Serogroups O26 and O111