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The development of a single histoplasmin suitable for use in immunodiffusion testing of a variety of human histoplasmosis sera depends not only upon the presence of both antigens, but on relative concentration of one to the other, and perhaps on the chemical characteristics of the H and M antigens of a given strain.

journal of clinical microbiology

Evaluation of Histoplasmin for the Presence of H and M Antigens: Some Difficulties Encountered in the Production and Evaluation of a Product Suitable for the Immunodiffusion Test