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Fregnan , G. B. (University of Wisconsin, Madison), D. W.Smith, and H. M. Randall . A mutant of a scotochromogenicMycobacterium detected by colony morphology and lipid studies. J. Bacteriol.83: 828–836. 1962.—A variant of a scotochromogenic strain ofMycobacterium is described. This mutant was recognized by studies of colony morphology and lipid content. Mycoside D was shown to be present in the smooth-Sy variety but absent in the rough-Ry variety. The rough-Ry variety appeared spontaneously from smooth-Sy, or by induction after prolonged incubation in a synthetic liquid medium or exposure to ultraviolet irradiation. This mutation is not reversible.

A MUTANT OF A SCOTOCHROMOGENIC MYCOBACTERIUM DETECTED BY COLONY MORPHOLOGY AND LIPID STUDIES