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ThesigE gene ofStreptomyces coelicolor A3(2) encodes an RNA polymerase sigma factor belonging to the extracytoplasmic function (ECF) subfamily. ConstructedsigE deletion and disruption mutants were more sensitive than the parent to muramidases such as hen egg white lysozyme and to the CwlA amidase fromBacillus subtilis . This correlated with an altered muropeptide profile, as determined by reverse-phase high-performance liquid chromatography analysis of lytic digests of purified peptidoglycan. ThesigE mutants required high levels of magnesium for normal growth and sporulation, overproducing the antibiotic actinorhodin and forming crenellated colonies in its absence. Together, these data suggest thatsigE is required for normal cell wall structure. The role of ςE was further investigated by analyzing the expression ofhrdD , which is partiallysigE dependent. ThehrdD gene, which encodes the ςHrdD subunit of RNA polymerase, is transcribed from two promoters,hrdDp 1 andhrdDp 2 , both similar to promoters recognized by other ECF sigma factors. The activities ofhrdDp 1 andhrdDp 2 were reduced 20- and 3-fold, respectively, insigE mutants, although onlyhrdDp 1 was recognized by EςE in vitro. Growth on media deficient in magnesium caused the induction of bothhrdD promoters in asigE -dependent manner.

journal of bacteriology

Evidence that the Extracytoplasmic Function Sigma Factor ς <sup>E</sup> Is Required for Normal Cell Wall Structure in <i>Streptomyces coelicolor</i> A3(2)

Evidence that the Extracytoplasmic Function Sigma Factor ς E Is Required for Normal Cell Wall Structure in Streptomyces coelicolor A3(2)

Evidence that the Extracytoplasmic Function Sigma Factor ς ^E Is Required for Normal Cell Wall Structure in Streptomyces coelicolor A3(2)