Open AccessDelayed expression of in vivo restriction activity following conjugal transfer of Escherichia coli hsdK (restriction-modification) genes
Author(s) -
Ainu Prakash-Cheng*,
Junichi Ryu
Publication year - 1993
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.175.15.4905-4906.1993
Subject(s) - biology , escherichia coli , gene , genetics , restriction site , restriction map , gene expression , microbiology and biotechnology , restriction enzyme , plasmid
Following conjugal transfer of the hsdK genes (hsdRK, hsdMK, and hsdSK) of Escherichia coli K-12, restriction activity was first detected only after approximately 15 generations, whereas modification activity was observed immediately. This sequential expression explains the establishment of hsdK genes in a nonmodified host and suggests regulation of restriction activity after conjugal transfer.