Open AccessSelectable mutant phenotypes of the extremely thermophilic archaebacterium Sulfolobus acidocaldarius
Author(s) -
Dennis W. Grogan
Publication year - 1991
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.173.23.7725-7727.1991
Subject(s) - sulfolobus acidocaldarius , biology , mutant , thermophile , novobiocin , genetics , phenotype , sulfolobus , microbiology and biotechnology , auxotrophy , archaea , bacteria , gene
As a first step toward developing the genetic potential of extremely thermophilic archaebacteria, mutant strains of Sulfolobus acidocaldarius were selected by plating cells directly on solid medium containing one of several growth inhibitors. Three spontaneous resistance phenotypes were observed (5-fluorouracil resistance, novobiocin resistance, and L-ethionine resistance), each at a different average frequency. Characterization of representative strains showed each of the three mutant phenotypes to provide a potentially useful genetic marker.