Open AccessGenetic suppression of a temperature-sensitive groES mutation by an altered subunit of RNA polymerase of Escherichia coli K-12
Author(s) -
Morimasa Wada,
Hiroshi Fujita,
Hiraku Itikawa
Publication year - 1987
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.169.3.1102-1106.1987
Subject(s) - groel , biology , groes , mutant , escherichia coli , microbiology and biotechnology , suppressor mutation , mutation , rna polymerase , gene , transduction (biophysics) , protein subunit , genetics , biochemistry
Temperature-resistant suppressor mutants were isolated from Escherichia coli mutant strain groES131(Ts). Phage P1-mediated transduction and a two-dimensional gel electrophoretic analysis of cellular proteins indicated that these suppressor mutants carry an additional mutation in either the groEL gene or the rpoA gene.