Open Accessgamma-Glutamyltranspeptidase from Escherichia coli K-12: formation and localization
Author(s) -
Hideyuki Suzuki,
Hidehiko Kumagai,
Tatsurokuro Tochikura
Publication year - 1986
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.168.3.1332-1335.1986
Subject(s) - periplasmic space , escherichia coli , biology , escherichia coli proteins , intracellular , enzyme , biochemistry , microbiology and biotechnology , biophysics , gene
Escherichia coli cells showed maximum activity of gamma-glutamyltranspeptidase (EC 2.3.2.2) when they were grown at 20 degrees C, 14% of maximum activity at 37 degrees C, and none at 43 degrees C. The enzyme activity of intact cells grown at 20 degrees C was stably maintained after the temperature was changed to 45 degrees C. The activity increased during the exponential phase, and maximum activity was found at stationary phase. Its intracellular localization in the periplasmic space was confirmed.