Chromosomal mapping in Erwinia carotovora subsp. carotovora with the IncP plasmid R68::Mu | Zendy

K. J. Forbes | Zendy; M. C. M. Pérombelon | Zendy

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Chromosomal mapping in Erwinia carotovora subsp. carotovora with the IncP plasmid R68::Mu

Author(s) -

K. J. Forbes,

M. C. M. Pérombelon

Publication year - 1985

Publication title -

journal of bacteriology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.652

H-Index - 246

eISSN - 1067-8832

pISSN - 0021-9193

DOI - 10.1128/jb.164.3.1110-1116.1985

Subject(s) - prophage , erwinia , biology , plasmid , mutant , genetics , chromosome , enterobacteriaceae , microbiology and biotechnology , gene , escherichia coli , bacteriophage

Conjugational gene transfer was established in Erwinia carotovora subsp. carotovora SCRI193 by using plasmid R68::Mu c+ to mobilize the chromosome into multiply mutant recipients. It was observed that although the plasmid alone mobilized markers randomly at a frequency of ca. 10(-5) chromosomal recombinants per donor, the presence of a Mu prophage on the chromosome of the donor increased the frequency of mobilization of markers adjacent to the prophage by up to 10-fold. Using this system it was possible to order 17 chromosomal mutations. The behavior of Mu in E. carotovora subsp. carotovora was also studied.

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