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Mutants of Bacillus subtilis with electrophoretic variants of ribosomal protein L1, L5, L9, or L11 were used to determine the order of the genes for these proteins by transformation experiments. The proteins are homologous with Escherichia coli proteins L1, L10, L12, and L11, respectively; using the gene locus designations based on this correspondence, we determined the order of the loci to be cysA-rplK-rplA-rplJ-rplL-rpoB. The order of the last five loci was identical to that of E. coli.

Order of ribosomal protein genes in the Rif cluster of Bacillus subtilis is identical to that of Escherichia coli