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A new method for extracting ribosomal ribonucleic acid (RNA) and polyribosomes fromSchizosaccharomyces pombe , a fission yeast, has been developed. Cells grown in the presence of 2-deoxyglucose were found to be more sensitive to the action of Glusulase; a short period of treatment (10 to 20 min) at 0 C was sufficient to convert the cells to osmotically sensitive structures. From these, ribosomal RNA and polyribosomes were readily prepared.

New Method for Extraction of Ribonucleic Acid and Polyribosomes from Schizosaccharomyces pombe