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Changes in the cell wall of a species ofFlavobacterium can be quantitated by measuring vancomycin adsorption. Treatment of the cells with ethylenediaminetetraacetic acid or tris(hydroxymethyl)aminomethane (or both) increased adsorption of the antibiotic.

journal of bacteriology

Quantitation of the Action of Ethylenediaminetetraacetic Acid and Tris(hydroxymethyl)aminomethane on a Gram-Negative Bacterium by Vancomycin Adsorption