Open AccessConformationally Correct Expression of Membrane-AnchoredToxoplasma gondiiSAG1 in the Primitive ProtozoanGiardia duodenalis
Author(s) -
Matthias Marti,
Yajie Li,
Peter O. Kohler,
Adrian B. Hehl
Publication year - 2002
Publication title -
infection and immunity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.508
H-Index - 220
eISSN - 1070-6313
pISSN - 0019-9567
DOI - 10.1128/iai.70.2.1014-1016.2002
Subject(s) - toxoplasma gondii , biology , giardia , heterologous , antigen , gene , virology , protozoa , giardia lamblia , microbiology and biotechnology , genetics , antibody
To explore the possibility of expressing membrane-anchored exodomains of heterologous surface antigens inGiardia , a chimeric construct containing theToxoplasma gondii SAG1 gene was made. TheGiardia system is shown here to provide a means of generating correctly folded chimeric surface proteins in a native and unmodified form.