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Vaccination of mice withEscherichia coli expressingBrucella Cu/Zn superoxide dismutase (SOD) [E. coli (pBSSOD)] induced a significant level of protection against virulentBrucella abortus challenge, although this level was not as high as the one reached withB. abortus vaccine strain RB51. In addition, vaccination withE. coli (pBSSOD) induced antibodies to Cu/Zn SOD and a strong proliferative response of splenocytes when stimulated in vitro with a thioredoxin-Cu/Zn SOD fusion protein.

infection and immunity

Vaccination with Live Escherichia coli Expressing Brucella abortus Cu/Zn Superoxide Dismutase Protects Mice against Virulent B. abortus