Open AccessPrecipitin Inhibition of Candida stellatoidea Antiserum with Oligosaccharides Obtained from the Parent Mannan
Author(s) -
William O. Mitchell,
H. F. Hasenclever
Publication year - 1970
Publication title -
infection and immunity
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.508
H-Index - 220
eISSN - 1070-6313
pISSN - 0019-9567
DOI - 10.1128/iai.1.1.61-63.1970
Subject(s) - mannan , antiserum , monosaccharide , biology , precipitin , polysaccharide , mannose , biochemistry , immune system , antigen , oligosaccharide , microbiology and biotechnology , immunology
Inhibition of immune precipitation with oligosaccharides obtained from Candida stellatoidea mannan has been used to provide more information about the haptenic groups of this serologically active polysaccharide. The oligosaccharides di-, tri-, tetra-, and a mixture of penta- and hexasaccharides were studied. The inhibitory activity of these materials was tested with two immune sera. With one serum, 0.8 mumole of the mixture of penta- and hexasaccharides inhibited the reaction by 87%, and, with the other serum, 0.4 mumole of the mixture inhibited the reaction by 99%. Monosaccharides were also tested with each antiserum and found to be noninhibitory. It is apparent that the mixture of oligosaccharides containing 5 to 6 mannose units was the most effective inhibitor. Since it is known that these oligosaccharides contain a predominance of alpha 1-2 linkages and lesser numbers of alpha 1-3 linkages, it is likely that these are important in the structural configuration of the antigenic determinants.