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Sterigmatocystin was produced by 59% ofAspergillus flavus cultures and by 16% ofA. parasiticus cultures. All sterigmatocystin-producing cultures of theA. flavus group also simultaneously produced aflatoxin orO -methylsterigmatocystin. Sterigmatocystin was produced byA. chevalieri, A. ruber , andA. amstelodami , species not previously reported to produce the compound. In 5-day-old chicken embryos, the no-effect level of toxicity of sterigmatocystin was between 1 and 2 μg/egg; the mean lethal dose was 5 to 7 μg; and 90 to 100% of the embryos were killed with 10 μg. Teratogenic effects and weight reduction were generally associated with nonlethal doses.

Production of Sterigmatocystin by Some Species of the Genus Aspergillus and Its Toxicity to Chicken Embryos