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The ventral surface of spawning salmonid fish was opened aseptically and the roe were removed aseptically. Roe obtained by using this technique were demonstrated to be sterile. Aseptic fertilization and incubation of eggs obtained in this manner resulted in the hatching of gnotobiotic salmonid fish.

Sterility of Salmonid Roe and Practicality of Hatching Gnotobiotic Salmonid Fish