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The indirect fluorescent-antibody technique was employed in an attempt to develop a rapid method of identification ofCorynebacterium vaginale . Six reference strains and ten clinical isolates selected on the basis of morphology and conventional biochemical tests were compared. Antisera were prepared in rabbits against the six reference strains. The most satisfactory antiserum was that prepared using strain 14018 grown diphasically (14018 Di) as the antigen. Certain of the antisera did exhibit a cross-reacting titer when reacted againstCorynebacterium diptheriae, Corynebacterium xerosis , orLactobacillus acidophilus . However, antisera adsorbed with these bacteria did not exhibit a significant decrease in titer when reacted against the homologous strain. Various other species ofCorynebacterium as well as species ofNocardia, Actinomyces, Hemophilus , andStreptococcus did not fluoresce with the antisera. A specific antiserum was prepared by adsorbing anti-14018 Di withL. acidophilus . The adsorption removed the cross-reacting antibody but did not affect the staining reaction withC. vaginale strains. All reference strains and clinical isolates characterized asC. vaginale gave a definite positive reaction with the adsorbed anti-14018 Di. The specificity of the reactions was assessed by adsorbing the antiserum with the homologous strain. The data suggest that the indirect staining method will be of value in the rapid presumptive identification ofC. vaginale .

Indirect Fluorescent-Antibody Method for the Identification of Corynebacterium vaginale