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Methods for the quantitation of bacteria in infected tissues must be rigidly standardized to insure uniformity of results. In this communication we report on a laboratory animal model for the study of surface wound infection and the development of a standardized method for the quantitative estimation of bacteria in infected surface wound tissue by mechanical tissue homogenization and serial dilution. Parallel comparative studies demonstrated that a moist-swab sampling procedure detected only 10% of the bacteria recoverable by a surface-wash procedure. Either tissue homogenization or surface-wash procedures recovered significantly more bacteria from contaminated surface wounds than were obtained by surface-swab sampling techniques.

Infected Surface Wound: an Experimental Model and a Method for the Quantitation of Bacteria in Infected Tissues