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A microbiological assay has been developed for chromomycin A3 , an antitumor antibiotic showing promise in human trials. The assay bacterium is a derived strain ofStreptococcus faecalis resistant to methotrexate. Studies with mice revealed that relatively high concentrations of this antibiotic were maintained in the blood, kidneys, and liver of mice after a single-dose intraperitoneal injection of the drug.

Chromomycin A 3 , an Antitumor Antibiotic: Tissue Distribution Studies in Mice as Measured by Microbial Assay