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To determine the most useful laboratory tests for the differentiation ofAchromobacter anitratus, Achromobacter lwoffii , andMoraxella duplex (DeBord's tribeMimeae ), 157 strains of these bacteria, isolated from clinical specimens, were examined for their morphological and biochemical characteristics. There were several differences between these nonfermentative, gram-negative diplococci:Moraxella was nonglucolytic in either infusion base or synthetic base, oxidase-positive, and sensitive to penicillin, whereasAchromobacter produced variable carbohydrate activity, and was oxidase-negative and resistant to penicillin.A. anitratus was distinguished fromA. lwoffii in that the former utilized infusion media containing either glucose or 10% lactose, whereas the latter did not. Both species utilized the same carbohydrates in a chemically defined medium, although the latter acted more sluggishly.

Morphological and Biochemical Differentiation of Achromobacter and Moraxella (Debord's Tribe Mimeae )