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Previous analysis of the Tra1 region of the conjugative element pRS01 fromLactococcus lactis subsp.lactis ML3 suggested that an origin of transfer (oriT ) was present. Deletion derivatives of this cloned Tra1 region were assayed for mobilization in the presence of the wild-type pRS01 element intrans . The pRS01oriT was localized to a 446-nucleotide segment in the intergenic region between open reading framesltrD andltrE . Sequence analysis of this region revealed a cluster of direct and inverted repeat structures characteristic oforiT regions associated with other conjugative systems.

An Origin of Transfer ( oriT ) on the Conjugative Element pRS01 from Lactococcus lactis subsp. lactis ML3