Identification of the Hepatitis C Virus RNA Replication Complex in Huh-7 Cells Harboring Subgenomic Replicons | Zendy

Rainer Gosert | Zendy; Denise Egger | Zendy; Volker Lohmann | Zendy; Ralf Bartenschlager | Zendy; Hubert E. Blum | Zendy; Kurt Bienz | Zendy; Darius Moradpour | Zendy

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Identification of the Hepatitis C Virus RNA Replication Complex in Huh-7 Cells Harboring Subgenomic Replicons

Author(s) -

Rainer Gosert,

Denise Egger,

Volker Lohmann,

Ralf Bartenschlager,

Hubert E. Blum,

Kurt Bienz,

Darius Moradpour

Publication year - 2003

Publication title -

journal of virology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.617

H-Index - 292

eISSN - 1070-6321

pISSN - 0022-538X

DOI - 10.1128/jvi.77.9.5487-5492.2003

Subject(s) - subgenomic mrna , biology , replicon , rna , viral replication , virology , rna dependent rna polymerase , hepatitis c virus , virus , microbiology and biotechnology , gene , genetics , genome

Formation of a membrane-associated replication complex, composed of viral proteins, replicating RNA, and altered cellular membranes, is a characteristic feature of plus-strand RNA viruses. Here, we demonstrate the presence of a specific membrane alteration, designated the membranous web, that contains hepatitis C virus (HCV) nonstructural proteins, as well as viral plus-strand RNA, in Huh-7 cells harboring autonomously replicating subgenomic HCV RNAs. Metabolic labeling with 5-bromouridine 5'-triphosphate in the presence of actinomycin D revealed that the membranous web is the site of viral RNA synthesis and therefore represents the replication complex of HCV.

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