Open AccessComparison of Two PCRs for Detection of Mycobacterium ulcerans
Author(s) -
Airton Guimaraes-Peres,
Françoise Portaels,
Pim de Rijk,
K. Fissette,
S. R. Pattyn,
J P Van Vooren,
Pierre-Alain Fonteyne
Publication year - 1999
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.37.1.206-208.1999
Subject(s) - 16s ribosomal rna , biology , polymerase chain reaction , mycobacterium ulcerans , ribosomal rna , nested polymerase chain reaction , gene sequence , gene , sequence analysis , virology , microbiology and biotechnology , genetics , medicine , pathology , disease
Two nested PCRs for the detection of Mycobacterium ulcerans were compared by using a collection of 65 clinical specimens. The first method amplifies the gene coding for 16S rRNA, and the second method amplifies a repetitive DNA sequence. The sensitivities of bacterioscopy, culture, 16S rRNA gene PCR, and repetitive-sequence PCR were 29, 34, 80, and 85%, respectively. Compared to the 16S rRNA gene PCR, the repetitive-sequence PCR was faster, easier to perform, and less expensive.
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