Open AccessComplete Nucleotide Sequence and Genetic Organization of the 210-Kilobase Linear Plasmid ofRhodococcus erythropolisBD2
Author(s) -
Christiane Stecker,
André Johann,
Christina Herzberg,
Beate Averhoff,
Gerhard Gottschalk
Publication year - 2003
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.185.17.5269-5274.2003
Subject(s) - biology , orfs , plasmid , transposition (logic) , genetics , gene , transposable element , nucleic acid sequence , horizontal gene transfer , open reading frame , insertion sequence , sequence (biology) , peptide sequence , genome , linguistics , philosophy
The complete nucleotide sequence of the linear plasmid pBD2 from Rhodococcus erythropolis BD2 comprises 210,205 bp. Sequence analyses of pBD2 revealed 212 putative open reading frames (ORFs), 97 of which had an annotatable function. These ORFs could be assigned to six functional groups: plasmid replication and maintenance, transport and metalloresistance, catabolism, transposition, regulation, and protein modification. Many of the transposon-related sequences were found to flank the isopropylbenzene pathway genes. This finding together with the significant sequence similarities of the ipb genes to genes of the linear plasmid-encoded biphenyl pathway in other rhodococci suggests that the ipb genes were acquired via transposition events and subsequently distributed among the rhodococci via horizontal transfer.
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