Identification and Characterization ofgshA, a Gene Encoding the Glutamate-Cysteine Ligase in the Halophilic ArchaeonHaloferax volcanii | Zendy

Liron Malki | Zendy; Michaela Yanku | Zendy; Ilya Borovok | Zendy; Gerald Cohen | Zendy; Moshe Mevarech | Zendy; Yair Aharonowitz | Zendy

Open Access

Identification and Characterization ofgshA, a Gene Encoding the Glutamate-Cysteine Ligase in the Halophilic ArchaeonHaloferax volcanii

Author(s) -

Liron Malki,

Michaela Yanku,

Ilya Borovok,

Gerald Cohen,

Moshe Mevarech,

Yair Aharonowitz

Publication year - 2009

Publication title -

journal of bacteriology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.652

H-Index - 246

eISSN - 1067-8832

pISSN - 0021-9193

DOI - 10.1128/jb.00297-09

Subject(s) - haloferax volcanii , biology , biochemistry , gene , dna ligase , cysteine , halobacterium , escherichia coli , microbiology and biotechnology , halophile , genetics , archaea , bacteria , enzyme

Halophilic archaea were found to contain in their cytoplasm millimolar concentrations of gamma-glutamylcysteine (gamma GC) instead of glutathione. Previous analysis of the genome sequence of the archaeon Halobacterium sp. strain NRC-1 has indicated the presence of a sequence homologous to sequences known to encode the glutamate-cysteine ligase GshA. We report here the identification of the gshA gene in the extremely halophilic archaeon Haloferax volcanii and show that H. volcanii gshA directs in vivo the synthesis and accumulation of gamma GC. We also show that the H. volcanii gene when expressed in an Escherichia coli strain lacking functional GshA is able to restore synthesis of glutathione.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research