Open AccessAcetylene Reduction (Nitrogen Fixation) by Pulp and Paper Mill Effluents and by Klebsiella Isolated from Effluents and Environmental Situations
Author(s) -
Roger Knowles,
R. Neufeld,
Stuart L. Simpson
Publication year - 1974
Publication title -
applied microbiology
Language(s) - English
Resource type - Journals
ISSN - 0003-6919
DOI - 10.1128/am.28.4.608-613.1974
Subject(s) - effluent , nitrogen fixation , anaerobic exercise , chemistry , xylose , pulp (tooth) , klebsiella , nitrogenase , sulfite , sewage , azotobacter , ammoniacal nitrogen , nitrogen , pulp mill , paper mill , food science , environmental chemistry , pulp and paper industry , biology , bacteria , fermentation , biochemistry , environmental engineering , environmental science , organic chemistry , escherichia coli , gene , engineering , pathology , physiology , medicine , genetics
High rates of acetylene (C2 H2 ) reduction (nitrogenase activity) were observed in woodroom effluent from a neutral sulfite semi-chemical mill under aerobic (up to 644 nmol of C2 H4 produced per ml per h) and under anaerobic (up to 135 nmol of C2 H4 produced per ml per h) conditions. Pasteurized effluent developed C2 H2 reduction activity when incubated under anaerobic but not under aerobic conditions. Activities were increased by addition of 0.5 to 3.0% glucose or xylose. Enrichment and enumeration studies showed that N2 -fixingAzotobacter andKlebsiella were abundant, and N2 -fixingBacillus was present. Of 129 isolates ofKlebsiella from pulp mills, lakes, rivers, and drainage and sewage systems, 32% possessed nitrogen-fixing ability.
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