Open AccessCell growth and catecholase production for Polyporus versicolor in submerged culture
Author(s) -
PAUL A. CARROAD,
C. R. Wilke
Publication year - 1977
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.33.4.836-839.1977
Subject(s) - polyporus , yield (engineering) , arrhenius equation , arrhenius plot , enzyme , chemistry , growth rate , biochemistry , biology , analytical chemistry (journal) , chromatography , activation energy , materials science , organic chemistry , mathematics , metallurgy , geometry
Cell growth and catecholase production for Polyporus veriscolor (ATCC 12679) were studied in mechanically agitated submerged culture, as functions of temperature. The exponential-phase growth rate exhibited a maximum at 28 degrees C. Over the range of 20 degrees C to approximately 30 degrees C, both cell mass and enzyme yield factors were constant. At higher temperatures (30 to 40 degrees C) cell mass yield factor decreased and enzyme yield factor increased. Specific respiration rate of P. versicolor was determined. Thermal deactivation of catecholase was investigated between 30 and 50 degrees C, and deactivation rates were fit to an Arrhenius rate expression.
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