Open AccessImipenem resistance in pseudomonas aeruginosa PAO: mapping of the OprD2 gene
Author(s) -
John P. Quinn,
Aldis Darzins,
D Miyashiro,
Steven Ripp,
Robert V. Miller
Publication year - 1991
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.35.4.753
Subject(s) - pseudomonas aeruginosa , imipenem , porin , microbiology and biotechnology , biology , gene , bacterial outer membrane , plasmid , antibiotics , pseudomonas , bacteria , escherichia coli , genetics
Carbapenem antibiotics have been shown to penetrate the outer membrane of Pseudomonas aeruginosa through a unique porin protein, OprD2. We mapped the OprD2 gene by conjugation using plasmid FP2 and by transduction using phage F116L. This gene maps between 71 and 75 min on the PAO1 chromosome.
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